Storage & Testing
This section contains information on the following topics: –
* The Animal Health Trust – who store DNA
* How to store DNA with the AHT
* DNA tests available for Soft-Coated Wheaten Terriers
Please scroll down to see more information on each of these topics.
DNA Storage at the Animal Health Trust (AHT)
Animal Health Trust (AHT) – Diagnostic Services UK
The AHT are an external Laboratory that has facilities to undertake testing and screening.
The AHT also stores DNA for Soft-Coated Wheaten Terriers.
Please consider having DNA from all Soft-Coated Wheaten Terriers that you own, or breed, stored at The Animal Health Trust (AHT).
Why is DNA storage important?
DNA is important for the future of the breed. ALL dogs, even those who are never bred from, are important. It is hoped that this DNA will enable researchers to find the deleterious (bad) mutations, which cause hereditary diseases.
What will this mean for breeders and owners?
In the future, if breeders have ALL health information, including if a dog has these deleterious mutations, it will enable the breeder to select ‘safer’ breeding combinations. Over time, the deleterious mutations may be eliminated from the gene pool.
How often do I have to collect DNA from my dog?
Just once, DNA can be stored indefinitely.
How to store DNA
First check that the breeder has not already stored DNA prior to the purchase of your puppy.
Storage can be achieved 2 ways:
* Cheek swab – which is simple and easy and does not require a visit to the Vet
* Blood sample – if your Wheaten Terrier is having blood taken for the recommended annual testing an additional amount of 5ml blood sample in an EDTA Tube can be sent to the AHT for DNA Storage purposes
DNA from family groups will be vital for future research and many breeders have DNA stored from each of the puppies in their litters, prior to the pups leaving for their new homes.
* Cheek swab – which is simple and easy and does not require a visit to the Vet.
* Blood sample – if you are having your puppies’ kidney function tested then an additional amount of 5ml blood sample in an EDTA Tube can be sent to the AHT for DNA Storage purposes.
Swab Kits and AHT forms are available from the Soft-Coated Wheaten Terrier Club of GB. Contact the Health Team at firstname.lastname@example.org
Or they can be downloaded here
The following items should be sent to the AHT:
* Cheek swab(s) or blood sample
* Completed AHT Form(s)
* 5 generation pedigree*
*The Soft-Coated Wheaten Terrier Club of GB can supply a 5 generation pedigree upon request. We will require your dog’s registered Kennel Club name, parent names, and date of birth of your dog to do this.
Who should I inform if I store DNA?
Your breeder and the Soft-Coated Wheaten Terrier Club of GB should be advised if you have your Wheaten Terrier’s DNA stored at the AHT.
DNA Tests for the Soft-Coated Wheaten Terrier
There are currently two DNA tests available for Soft-Coated Wheaten Terriers. These are: –
* PLN-Associated Variant Gene Test (PLN)
* Degenerative Myelopathy (DM)
The PLN -Associated Variant Gene Test can be tested at either Penn Gen in the USA, or through Laboklin Laboratories in the UK.
DM can be tested through Laboklin Laboratories in the UK.
When taking the DNA test swab samples, it is VERY IMPORTANT that you follow the instruction guides relevant to each laboratory. They may not be the same.
If you wish to submit test swabs for either PLN and/or DM through Laboklin Laboratories, the special Club endorsed submission forms, instructions and swabs can be obtained from the Health Team Please email them at the following link – Health Team.
(Note – The Soft-Coated Wheaten Terrier Club of GB has negotiated a special rate for our Members who wish to test for both PLN & DM through Laboklin Laboratories – you MUST use the special Club endorsed submission forms to do this.)
If you wish to submit test swabs for PLN through Penn Gen, then please contact them directly for their instructions and forms at the following link – Penn Gen . Test swabs can be obtained from the Club’s Health Team (link above).
The testing laboratory will contact you with your dog/litter result and this result is confidential between you and the Laboratory used.
As each laboratory provides results using their own definitions, there is a table showing how each of their reporting definitions compares with the other, as well as some more commonly used terms that are in use. This table can be found at the following link – PLNVariantGeneTable
It is recommended that you send copies of the results to the Breeder, and if the Breeder or Owner chooses they can share them with The SCWT Club of GB.
What does “Clear by Parentage” mean?
The genetic status of both the sire and dam will influence their offspring.
You may hear the term “Clear by Parentage” used. This means that if both parents have been DNA tested as being clear for a disease, it is declared offspring are clear by virtue of the fact that the parents both tested clear. However, there are a few conditions to clear by parentage.
As mentioned, both sire and dam must have tested clear, and those documented test results from the testing laboratory can then be recorded on the SCWT Endowment Database at http://scwtdb.org/ .
(For information on how to have your Wheaten Terrier’s results included on the SCWT Endowment Database please read the following letter from Molly O’Connell, who is the Chair of Health for the SCWTCA. To open the letter in a new page, click on the following link – How to include test results on SCWT Endowment Database)
Therefore, your dog is now cleared by parentage for that disease. However, the results will only be shown as ‘clear by parentage for one generation’. This is due to the possibility of new mutations, or as yet undiscovered gene mutations developing. DNA based screening is an evolving science and is subject to change as technology and science advance.
Breeders should also read ‘UNDERSTANDING BREEDING COMBINATIONS – A Breeders’ Tool’ (an update January 2014) which can be found at the following link – Breeding Combinations
Further Information about PLN and DM are available on our health pages and in the Soft-Coated Wheaten Terrier Club of GB Health Booklet at the following link – SCWT Health Booklet